| Airline Name | ICAO | IATA | Callsign | Country |
| NACAM - National Airways Cameroon | 9O | Cameroon | ||
| Nacional Transportes Aereos | NCT | Brazil | ||
| Nader Omer Al-Bazz | Jordan | |||
| Nakanihon Airlines (NAL) | NV | Japan | ||
| Namib Air | Namibia | |||
| Nas Air | NCM | P9 | Mali | |
| Nas Air | KNE | XY | NAS EXPRESS | Saudi Arabia |
| NASA | United States | |||
| NasAir | NAS | UE | NASAIRWAYS | Eritrea |
| Nationair | NXA | NX | Canada | |
| National Air Charter | NSR | Indonesia | ||
| National Air Services (NAS) | KNE | XY | Saudi Arabia | |
| National Airlines | NCN | N4 | Chile | |
| National Airlines | NAN | NATION AIR | United States | |
| National Airlines | NAL | NA | NATIONAL | United States |
| National Airlines | ROK | N7 | RED ROCK | United States |
| National Jet Express | JTE | Australia | ||
| National Jet Italia | Italy | |||
| National Jet Systems | NJS | NC | Australia | |
| National Private Air Transport | Unknown | |||
| Nationale Regionale Transport | Gabon | |||
| Nations Air Express | United States | |||
| Nationwide Airlines | NTW | CE | NATIONWIDE | South Africa |
| Naturelink Charter | NRK | South Africa | ||
| Nav Canada | NVC | Canada | ||
| NAYSA Aerotaxis | NAY | Spain | ||
| Nayzak Air Transport | NZA | M4 | Libyan Arab Jamahiriya | |
| NCB | United States | |||
| Necon Air | NEC | 3Z | Nepal | |
| Neos | NOS | NO | Italy | |
| Netjets International | NJT | United States | ||
| Network Aviation | Australia | |||
| New Axis Airways | AXY | 9X | AXIS | France |
| New York Airways | NYA | NY | United States | |
| New Zealand AC | New Zealand | |||
| New Zealand Air Defence Force | KRC | New Zealand | ||
| NewAir (Airservice) | NAW | 8L | NEWDAN | Denmark |
| News Corporation America | United States | |||
| NFD Luftverkehrs AG | NFD | NS | Germany | |
| Nicaraguenses de Aviacion (NICA) | NIS | 6Y | Nicaragua | |
| Nicon Airways | NCN | NICON AIRWAYS | Nigeria | |
| Niger Government | Niger | |||
| Nigeria Airways | NGA | WT | Nigeria | |
| Nigerian Air Force | NGR | Nigeria | ||
| Nigerian Global | NGI | Nigeria | ||
| Niki | NLY | HG | FLYNIKI | Austria |
| Nile Air | NIA | HAPI AIR | Egypt | |
| Nippon Cargo Airlines | NCA | KZ | Japan | |
| Nok Air | DD | Thailand | ||
| Nolinor Aviation | NRL | Canada | ||
| Nord-Flyg | NEF | NORDEX | Sweden | |
| Nordair | ND | Canada | ||
| Nordeste Linhas Aereas | NES | JH | Brazil | |
| Nordic Airlink (FlyNordic) | NDC | LF | Sweden | |
| Nordic East Airways | ELN | N7 | Sweden | |
| Nordic European Airlines | NOD | DJ | Sweden | |
| Nordic Leisure | NRD | 6N | NORTH RIDER | Sweden |
| Nordic Regional | NRD | 6N | NORTH RIDER | Sweden |
| Nordstress Australia | Australia | |||
| Nordwind Airlines | NWS | NORDLAND | Russian Federation | |
| Norfolk Air | O7 | Norfolk Island | ||
| Norfolk Island Airlines | NIA | UG | Australia | |
| Norfolk Jet Express | YE | Norfolk Island | ||
| Norlink (SAS Commuter) | SAS | SK | SCANDINAVIAN | Norway |
| Norontair | NOA | Canada | ||
| Norse Air | NRX | South Africa | ||
| North American Airlines | NAO | NA | United States | |
| North Atlantic Air | NAT | United States | ||
| North Caribou Flying Service | NCB | Canada | ||
| North Central Airlines | NC | United States | ||
| North Pacific Aviation | United States | |||
| Northeast Airlines | DBH | NS | China | |
| Northeast Airlines | Swaziland | |||
| Northeast Express Regional Airlines (Northwest Airlink) | NEE | NORTHEAST | United States | |
| Northeastern International Airways | United States | |||
| Northern Air Cargo | NAC | NC | United States | |
| Northwest Airlines | NWA | NW | NORTHWEST | United States |
| Northwest Airlink (Express Airlines) | United States | |||
| Northwest Airlink (Pinnacle Airlines) | FLG | 9E | United States | |
| Northwest Orient Airlines | NWA | NW | United States | |
| Nortjet | ENJ | EL | Spain | |
| Nortland Air Manitoba | NAM | 7N | Canada | |
| Norway Airlines Charter | NOS | Norway | ||
| Norwegian Air Shuttle | NAX | DY | NOR SHUTTLE | Norway |
| Nouvelair Tunisie | LBT | BJ | NOUVELAIR | Tunisia |
| Nova Airline | NOV | NOVANILE | Sudan | |
| Novair | NVR | 1I | NAVIGATOR | Sweden |
| Novair International | NGK | EN | United Kingdom | |
| Nuernberger Flugdienst (NFD) | Germany | |||
| NWT Air | NWT | NV | Canada |